I'm an audiophile myself, and yes, I can hear the difference between quality of lossless (for ex. APE or FLAC) and MP3 at 320 kbit/s without headphones. Occasionally I notice huge drops of sound quality in fansubs. The last time it bothered me was when the audio was encoded at 112 kbit/s (also, the ED was truly great, so it bothered me a lot
). But when watching anime fansubs I focus on other stuff whereas listening to music I can only concentrate on that, so audiophilia and fansubbing... I really don't see the point.