I did realize that Kayaba got his HP reduced (thinking it was due to lifting his immortal status), but with the absolute absence of explanations from him or numerical value that literally disappeared ever since episode 3-4 (can't remember), it is absolutely impossible to conclude with the anime alone than a single hit would be game over for any of them, especially that HP bar should never be counted by the %/appearance but by their current/MAX respective value, especially with a game that involve obvious difference in term of stats.
Oh well, another "compliment" for the studio.
Side note: it makes Kuradeel stabs even weaker than it already look